© 2019 by Specifica Inc. 

Our Publications

Specifica Publications

Selection and characterization of Fc epsilon R1 phospho-ITAM specific antibodies

Nileena Velappan, Avnika Mahajan, Leslie Naranjo, Prianka Velappan, Nasim Andrews, Nick Tiee, Subhendu Chakraborti, Colin Hemez, Tiziano Gaiotto, Bridget Wilson, Andrew Bradbury

2019, MAbs: 1-13. doi: 10.1080/19420862.2019.1632113

Recombinant antibodies against mycolactone

Leslie Naranjo, Fortunato Ferrara, Nicolas Blanchard, Caroline Demangel, Sara D'Angelo, M. Frank Erasmus, André TeixeiraAndrew Bradbury

2019 Toxins (Basel) 11(6). pii: E346. doi: 10.3390/toxins11060346

Rapid purification of billions of circulating CD19+ cells directly from leukophoresis samples

Fortunato Ferrara, Martin Kolnik, Sara D'Angelo, M. Frank Erasmus, André Teixeira, Daniela Vorholt, Andrew Bradbury

2018 N Biotechnol 46: 14-21. doi: 10.1016/j.nbt.2018.05.006

Many routes to an antibody heavy-chain CDR3: necessary, yet insufficient for specific binding

Sara D'Angelo, Fortunato Ferrara, Leslie Naranjo, M. Frank Erasmus, Peter Hraber, Andrew Bradbury

2018 Frontiers in Immunology 9 doi: 10.3389/fimmu.2018.00395

Selection of phage displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications

Sara D'Angelo, Fernanda Staquicini, Fortunato Ferrara, Daniela Staquicini, Geetanjali Sharma, Christy Tarleton, Huynh Nguyen, Leslie Naranjo, Richard Sidan, Wadih Arap, Andrew Bradbury, Renata Pasqualini

2018 JCI Insight 3(9) doi: 10.1172/jci.insight.98305

When monoclonal antibodies are not monospecific: hybridomas frequently express additional functional variable regions

Andrew Bradbury, Nathan Trinklein, Holger Thie, Ian Wilkinson, Atul Tandon, Stephen Anderson, Catherine Bladen, Brittany Jones, Shelly Force Aldred, Marco Bestagno, Oscar Burrone, Jennifer Maynard, Fortunato Ferrara, James Trimmer, Janina Görnemann, Jacob Glanville, Philipp Wolf, Andre Frenzel, Julin Wong, Xin Yu Koh, Hui-Yan Eng, David Lane, Marie-Paule Lefrance, Mike Clark, Stefan Dübel

2018 MAbs 10 1-19 doi: 10.1080/19420862.2018.1445456

Selected Prior Publications by our Scientists

Quaglia, S. et al. A Functional Idiotype/Anti-Idiotype Network Is Active in Genetically Gluten-Intolerant Individuals Negative for Both Celiac Disease-Related Intestinal Damage and Serum Autoantibodies. Journal of Immunology, doi:10.4049/jimmunol.1800819 (2019).

Phipps, M. L. et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11, e0160940, doi:10.1371/journal.pone.0160940 (2016).

Jones, T. D. et al. The INNs and outs of antibody nonproprietary names. MAbs 8, 1-9, doi:10.1080/19420862.2015.1114320 (2016).

Erasmus, M. F. et al. Dynamic pre-BCR homodimers fine-tune autonomous survival signals in B cell precursor acute lymphoblastic leukemia. Science signaling 9, ra116, doi:10.1126/scisignal.aaf3949 (2016).

Glanville, J. et al. Deep sequencing in library selection projects: what insight does it bring? Curr Opin Struct Biol 33, 146-160, doi:10.1016/j.sbi.2015.09.001 (2015).

Ferrara, F., Kim, C. Y., Naranjo, L. A. & Bradbury, A. R. Large scale production of phage antibody libraries using a bioreactor. MAbs 7, 26-31, doi:10.4161/19420862.2015.989034 (2015).

Bradbury, A. R. & Pluckthun, A. Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents. Protein Eng Des Sel 28, 303-305, doi:10.1093/protein/gzv051 (2015).

Bradbury, A. & Pluckthun, A. Reproducibility: Standardize antibodies used in research. Nature 518, 27-29, doi:10.1038/518027a (2015).

D'Angelo, S. et al. From deep sequencing to actual clones. Protein Eng Des Sel 27, 301-307, doi:10.1093/protein/gzu032 (2014).

D'Angelo, S. et al. The antibody mining toolbox: an open source tool for the rapid analysis of antibody repertoires. MAbs 6, 160-172, doi:10.4161/mabs.27105 (2014).

Close, D. W. et al. Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes. BMC Microbiol 13, 270, doi:10.1186/1471-2180-13-270 (2013).

D'Angelo, S. et al. Profiling celiac disease antibody repertoire. Clin Immunol 148, 99-109, doi:10.1016/j.clim.2013.04.009 (2013).

Ferrara, F., Naranjo, L. A., D'Angelo, S., Kiss, C. & Bradbury, A. R. Specific binder for Lightning-Link(R) biotinylated proteins from an antibody phage library. Journal of immunological methods 395, 83-87, doi:10.1016/j.jim.2013.06.010 (2013).

Ferrara, F. et al. Using phage and yeast display to select hundreds of monoclonal antibodies: application to antigen 85, a tuberculosis biomarker. PLoS One 7, e49535, doi:10.1371/journal.pone.0049535 (2012).

Ferrara, F., Listwan, P., Waldo, G. S. & Bradbury, A. R. Fluorescent labeling of antibody fragments using split GFP. PLoS One 6, e25727, doi:10.1371/journal.pone.0025727 (2011).

D'Angelo, S. et al. Filtering "genic" open reading frames from genomic DNA samples for advanced annotation. BMC genomics 12 Suppl 1, S5, doi:10.1186/1471-2164-12-S1-S5 (2011).

Bradbury, A. R. M., Sidhu, S., Dübel, S. & McCafferty, J. Beyond natural antibodies: the power of in vitro display technologies. Nature Biotechnology 29, 245-254, doi:10.1038/nbt.1791 (2011).

Velappan, N. et al. A comprehensive analysis of filamentous phage display vectors for cytoplasmic proteins: an analysis with different fluorescent proteins. Nucleic acids research 38, e22, doi:10.1093/nar/gkp809 (2010).

Di Niro, R. et al. Rapid interactome profiling by massive sequencing. Nucleic acids research 38, doi:10.1093/nar/gkq052 (2010).

Velappan, N., Sblattero, D., Chasteen, L., Pavlik, P. & Bradbury, A. R. Plasmid incompatibility: more compatible than previously thought? Protein Eng Des Sel 20, 309-313, doi:10.1093/protein/gzm005 (2007).

Di Niro, R. et al. Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models. BMC Biotechnol 7, 46-55 (2007).

Ayriss, J., Woods, T., Bradbury, A. & Pavlik, P. High-throughput screening of single-chain antibodies using multiplexed flow cytometry. J Proteome Res 6, 1072-1082, doi:10.1021/pr0604108 (2007).

Kehoe, J. W. et al. Using phage display to select antibodies recognizing post-translational modifications independently of sequence context. Mol Cell Proteomics 5, 2350-2363, doi:10.1074/mcp.M600314-MCP200 (2006).

Chasteen, L., Ayriss, J., Pavlik, P. & Bradbury, A. R. Eliminating helper phage from phage display. Nucleic acids research 34, e145, doi:10.1093/nar/gkl772 (2006).

Di Niro, R. et al. Characterizing monoclonal antibody epitopes by filtered gene fragment phage display. The Biochemical journal 388, 889-894, doi:10.1042/BJ20041983 (2005).

Siegel, R. W., Velappan, N., Pavlik, P., Chasteen, L. & Bradbury, A. Recombinatorial cloning using heterologous lox sites. Genome Res 14, 1119-1129, doi:10.1101/gr.1821804 (2004).

Zacchi, P., Sblattero, D., Florian, F., Marzari, R. & Bradbury, A. Selecting open reading frames from DNA. Genome Research 13, 980-990, doi:10.1101/gr.861503 (2003).

Lou, J. et al. Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries. Journal of immunological methods 253, 233-242, doi:10.1016/S0022-1759(01)00385-4 (2001).

Sblattero, D. & Bradbury, A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat Biotechnol 18, 75-80, doi:10.1038/71958 (2000).