Antibody Discovery & Affinity Maturation
Specifica uses three powerful coordinating technologies to select the best antibodies. Preliminary selections are carried out from our proprietary phage antibody display libraries to generate an initial pool of potential antibody leads. Yeast display is then used to isolate a population in which all antibodies bind the target, or which show desired binding properties, such as species cross-reactivity. This approach combines the advantages of both approaches. Finally, next generation sequencing is used to rank antibodies according to different properties of interest, including affinity, or specificity.
Specifica uses a number of different diversification approaches including light chain shuffling, error prone PCR and targeted mutations prior to selecting higher affinity antibody variants by yeast display.