Affinity Maturation & Antibody Optimization

Improve affinity without losing the properties that matter

Advance promising antibodies with an affinity maturation and optimization strategy designed around your molecule and program goals. Specifica applies controlled library design and in vitro selection to improve binding performance while maintaining focus on specificity and developability.

• • • 10- to 1,000-fold improvement in affinity while preserving the epitope-binding characteristics

    • Controlled selection designed to iteratively explore the vast diversity of the human antibody repertoire for better variants

    • A focused path for existing antibody leads, resulting in a set of variants not only with improved binding but also with retained or even improved developability

Our platform improves antibodies from both in vitro and in vivo platforms that have the desired biological activity but need additional optimization. 

• Define the optimization objective

  • Align on affinity, specificity and other requirements
    
    

• Build focused maturation libraries

  • Resample deeply characterized LCDR1-2-3, HCDR1-2 diversity, matching it to the variable domain of family of the parental close.
  • Bioinformatically analyze the parental clone to identify and remove sequence liabilities.

  • Leave HCDR3 untouched to prevent drift, or introduce mutational scanning if desired.
    
    

• Select and characterize improved variants

  • Apply in vitro selection and screening against the desired profile.

Our process ensures the exploration of a diversity up to 1018 CDR combinations while preserving epitope recognition.