Gen 2 Platform
Specifica's Generation 2 Libraries are natural antibody libraries created from the rearranged antibody variable genes found in natural B lymphocytes. At least 500 million B cells derived from at least ten donors are usually used as source materials. In general, all germline V genes tend to be represented in proportions reflecting their natural abundance, but that can be varied to some degree, during the library construction process. You can expect hundreds of different antibodies, many of which will have affinities less than 20 nM.
The main advantage of Generation 2 libraries lies in the high germline diversities found in natural sources, which can lead to antibodies with unusual biological properties. However, as these libraries harvest the natural diversity found in the donor pool, they also contain antibodies that may require downstream optimization.